Presence and absence of persistent soil seed banks or seedling banks are one of the features, among others, which imply the restoration potential of a forest or plant community. As a result, soil seed bank research is always of great interest. Can any body recommend me the volume of the soil sufficient (consider larger number small-sized samples vs small number of large-sized samples) for soil seed bank to decide the restoration potential of an area? I am asking this question because I believe the smaller the sample could influence our judgement.