How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

Similarity between these two waveform? Similarity in terms of Shape?

Hi, These are the outputs generated by a system. Green-colored (nr) is desired output, while the system generates Red-colored (nf). I also have a dataset in CSV file. For this waveform, What...

21 January 2021 929 2 View

Which kind of GPC solvent can be used to dissolve both lipid and salt?

I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).

14 January 2021 3,805 2 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

Is it possible to deposition a large area dielectric layer(mm scale) on the wet transferred graphene?

I want to deposition a dielectric layer on top of the CVD wet transferred graphene. The purpose of the dielectric layer is to uniformly cover the graphene and enable the top-gate modification of...

14 December 2020 7,693 2 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

What are the important topics in the field of Artificial Intelligence in Business world?

Dear All, I am doing my MS in Electronic Businesses. And I am interested in the AI field and its applications for businesses, its benefits, impacts, etc. What kind of scientific research dominate...

26 February 2021 8,816 6 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

How can we improve AI in detecting fraudulent activities on digital payments?

I am conducting a study on the effectiveness of AI towards detecting and averting digital payment fraud. What are the popular AI techniques being adopted in mitigating digital fraud and how could...

21 February 2021 3,888 4 View

Can anyone suggest a topic for the Impact of AI and ML in the financial institutions?

I am currently doing my Master Thesis in FinTech and I am thinking of doing my project on the impact of AI and ML on the financial industry but as the impact of these technologies on the industry...

21 February 2021 7,128 3 View

Why at farmer level Cow/Buffalo are not done artificial insemination just after 2 month for yearly calving?

Physiologically in cow, oestrous heat again goes on the peak after 2 months but locally this is not observed and AI is after two months but done after at least 6 months.

17 February 2021 685 4 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View