Breaking the dormant phase in the seeds

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to convert protein percentage into kg/ha?

I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.

11 July 2024 9,045 4 View

How can I determine the percentage of cell viability of cells seeded in 96 well plate ?

I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...

10 July 2024 7,145 2 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

Zhang Keliang

After a magnetic or ultraviolet rays, germination percentage of some seeds could be increased, but the reason is unknown,

There;s some example

https://www.researchgate.net/publication/328008763_A_review_of_the_seed_biology_of_Paeonia_species_Paeoniaceae_with_particular_reference_to_dormancy_and_germination?_sg=kqYN7nUmP82e8kSxOPoAA__H5eC0JgR27LxblVB-fAW0eA8hUbWAz2A1Bb9zh9X5EuxjE4sj_P27cxtVfnWHaXnNJzAxcHvGB_l6j3Of.iBO6Kl_4AQRkm-lnIUpx85pNi2euxTa3ONJDSCTCSEZBJnDJRmSRRaw3dkxMk2GReawvIiDIqIByTT4amchGqQ

Mohammad Sarraf

Dear ايناس الفرحة

have a look, I hope it will be useful

ايناس الفرحة

Article Magnetic Field (MF) Applications in Plants: An Overview

Andrew Paul McKenzie Pegman

You would need to do biochemical tests and compare treated and untreated replicates