Hi everyone.
I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain positive control. Any possible reasons to have that issue??
thanks!
My BrdU protocol:
DNA Hydrolysis with 2N HCL at 37oC for 30min, and the neutralised with 0.1M Borate buffer for 10min, and washing with 1X PBS and go for the normal immunostaining step.