Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the best solution for counter electrode for perovskite solar cells at room conditions?

I'm investigating transparent counter electrode for FTO/PEDOT:PSS/MAPI3/PCBM layered perovskite solar cell. In my research lab, currently i have a glove box with N2 gas as a carrier. I'm goning...

21 December 2020 6,504 3 View

My status has changed from "Awaiting Reviewer Score" to Awaiting AE Decision". Could the Associate Editor reject the manuscript at this stage?

Otherwise what this status means. If the Editor has received the comments from reviewer then why he is not sending it to Authors. As 10 days already spent till the status changed.??

18 November 2020 6,252 4 View

How to adapt a mature Questionnaire?

I am looking forward to seek some help regarding adaptation of mature questionnaires. I am new to quantitative studies and wants to use some demographic in my questionnaire, is it necessary to add...

08 November 2020 7,146 5 View

Is there a way to isolate nuclei without purifying cytoplasmic protein aggregates?

Hello, I am trying to isolate nuclei from HEK293T cells expressing a protein that aggregates in the cytoplasm, however when I run my samples in western blot the cytoplasmic protein is strongly...

02 November 2020 9,541 3 View

How can i fill zinc oxide or titanium oxide into etched pattern on glass substrate?

Hi! I need zinc oxide or titanium oxide to be filled into etched area on glass sbstrate. Etched are is being photolithography processed and being dry etched. Material must not present around the...

25 October 2020 152 7 View

How to get the most out of your qPCR data?

23 October 2020 7,537 3 View

Do you think that the e-learning that took place after the COVID 19 crisis was fair for students to evaluate their knowledge?

The significance of the question is to know the extent of accuracy in evaluating students.

19 September 2020 3,663 13 View

How to calculate reliability for a newly launched system?

We have a hydraulic system (water collector and distributor) which is newly started, how can we calculate its reliability?

07 September 2020 6,619 2 View

Neonatal Renal dose adjustment! Guidelines or the reference that is most authentic in clinical practice.??

In the infants to the adult age group, we have been given a concrete guideline for dose adjustment on the basis of CKD-Stages or creatinine clearance. this pathway is missing in neonates but...

07 September 2020 1,731 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View