How should we define the initial concentration to start the experiment? by literature or by our own consideration based on recorded concentration in studied areas? And is it different (initial concentration) if we are using dead or fresh live samples?
This will depend upon the concentration usually found in the wastewater. For this you are suggested to go for literature review and the metal ion under consideration. Your selection method will also depend upon the method for analysis of residual concentration of metals. As far as last part of the your question is concerned, It will depend upon the load of seaweed rather than initial concentration which you can increase of decrease depending upon the two types of biomass you have mentioned.
It is based on preliminary trials that we perform using one factor at a time approach.
Sorry for my late response, thank you all. I've start my test. As Sir @Harminder sigh said, load of seaweed for each test do concern more rather than concentration of medium. I'm testing different weight now for life and dead samples. It's interesting to see how each weight reacts differently.
@Abdul M Ziyath Thank you sir, really helpful. I do take note on the concentration range of my study areas as i've done the research on monitoring previously and will apply that in my current test.
@selvaraju Sivamani Thanl you! yes, i've listed many factors now (weight of samples, pH, RPM and etc) for preliminary.
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