I recently using the Bio-Rad droplet PCR kit for a qPCR application, However, I realized that the generated droplets' viscosity increased a lot after thermal cycling (40X).
Then, I replaced the Bio-Rad oil system using Novec 7500 +5% Picosurf, the viscosity did not increase.
So if use entire kit from Bio-rad the droplet viscosity increased after Thermal cycling
If use Novec 7500 +Bio-rad reagent supermix for the aqueous phase, no increase in viscosity.
Does anyone know what makes the viscosity increased?
I have difficulty to merge these high viscosity droplets with another droplets library for downstream assay
Hi,
I don't know answer to your question, but we wonder if you have measured the viscosity with a viscometer? We need that value for the droplet microfluidics calculations for our manuscript, but Bio-Rad does not provide the viscosity values, not even by request.
Anyone used viscometer for Bio-Rad oils, please help!
Best,
Päivi
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