Salam dears all,
Please, according to your experiences, what is the best method for dosing flavonoids in plant extracts. for me, I used the known method of AlCl3 ... but sometimes it presents bizzare results. in some of my essays, some of my colleagues' essays and according to some articles, the flavoinoides content according to this method seems superior to that of total phenolic compounds !! what is explained in literary by the low specificity of reagent that can interfere with other compounds such as proteins ...So, I need a more specific protocol well tried.
thanks for your helps!
Total Phenolic and Flavonoid Content and Antioxida
January 2018
Jinting AzieanaMohd Noor ZainonAbdullah NorihamRohana Mat NorRohana Mat Nor
Plant Flavonoid Content Modified by Domestication
August 2017Environmental Entomology 46(5)
DOI: 10.1093/ee/nvx126
Manuel Alejandro Chacón-FuentesManuel Alejandro Chacón-FuentesLeonardo ParraLeonardo ParraMarcelo LizamaMarcelo LizamaShow all 6 authorsAndres QuirozAndres Quiroz
This method is more qualitative rather than quantitative. For more sensitive detection and quantification use HPLC.
yes I agree with Muhammad Nadeem, For sensitive detection and quantification of flavonoid, use HPLC.
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