I have different polar and non polar plant extracts (Methanol, Acetone, Chloroform, Pet ether) and i want phytochemical screening by GC-MS .then should i go for HPLC before going for GC-MS or directly gcms would help me .
Can anybody provide me the protocol for the sample preparations for GC-MS from the plant extracts not essential oil.
Not necessary as both had different principles. For GC-MS, ideally you sholuld know the B.P. of your sample as well as solvent in which you had prepared sample.
Regards
Abhishek Sharma
phytochemical screening by GC-MS ?
What are the compounds of your interest? Are they volatile? If not GC-MS will not help you. You can try derivatization for GC-MS or start to think about HPLC or HPLC-MS
regards,
G
Kindly follow link below for some suggestions
https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/gas-chromatography-mass-spectrometry-gc-ms-information/gc-ms-sample-preparation.html
I agree with Dr. Abhishek Sharma as well as Dr. Grzegorz Boczkaj as only volatile compounds are detectable on GC-MS.
In my opinion, you can go directly to 'screen' for phytochemicals. But to be able to us GC-MS, you need a pure single compound for your data to make sense. otherwise you will not know where the masses are coming from.
ie. HPLC will help you to isolate once compound from another, then you can take the purified compounds and get them each into MS systems (GC-MS if volatile compounds, LC-MS would do better for non volatile ones)
All the best
From the 4 different extracts, chloroform and Pet ether might likely provide volatile compounds and therefore you may analyse on GC-MS and see what you obtain. But Acetone and MeOH extracts will definitely provide good LC profile if you use LC-MS, for instance ESI-MS. If your chloroform and Pet ether extracts are soluble in acetonitrile, you may also try ESI-MS on them.
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