Does anyone have/know any data on how the volume of recovery media affects things for electroporation of bacteria?
I see large ranges. For electroporating, eg, 200 uL concentrated bacteria, sometimes it's 1 mL (5 volumes), sometimes it's 10 mL (50 volumes). And some protocols hint that it matters a lot due to something about osmolarity. Eg, one protocol mentions recovery *must* be 10 volumes for "osmotic" reasons.
I'm also hoping to hear anything about duration of recovery too. Specifically for bacteria that have doubling times greater than 1 hr. I know for ecoli it's always 1 hr recovery.
Typically the recovery volume will be 1 ml that is 5-20 volumes, with comp cells of varies (50ul to 200ul) volumes.
I don't think that further increase of recovery media to 5-10 ml volume will enhance the transformants number.
For the bacteria with doubling time >1hr, the enrichment duration should be at least 24 hr.
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