Hello,
Did someone can help me to identify some kind of bacteria I seen in Scanning Electron Microscopy on one of my samples? Any ideas is welcomed!
Thank you in advance,
Monica
Dear Monica,
From the image the size of the bulged bodies (possibly any microbe) is around 5 micrometer. While size of most bacterial cells are around 0.5-1 micrometer. Thus, most probably it is a yeast/fungus, try to look back on the protocol and methods you used, from where you may anticipate the microbe. Moreover, if you have sample, you can cultivate them in lab and check for its identity. The best way would be to do a 16S sequencing directly from the sample (provided it is only one type/species of microbe).
You can leave your specific question for better understanding. I hope it would be of help.
Regards
Prasun
You can culture to any bacteriological broth, then make gram's staining to differentiate them either gram positive or negative, shape, the size of bacteria then select specific agar media to culture and purify the bacteria. This is very simple and initial step to identify bacteria in the sample. If you want to know details you can ask again with a specific question. Best of luck.
Morphology; physiology; ecology; phylogeny.
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Things are what they do.
Dear Monica,
From the image the size of the bulged bodies (possibly any microbe) is around 5 micrometer. While size of most bacterial cells are around 0.5-1 micrometer. Thus, most probably it is a yeast/fungus, try to look back on the protocol and methods you used, from where you may anticipate the microbe. Moreover, if you have sample, you can cultivate them in lab and check for its identity. The best way would be to do a 16S sequencing directly from the sample (provided it is only one type/species of microbe).
You can leave your specific question for better understanding. I hope it would be of help.
Regards
Prasun
Dear monica, I aggre with Dr. Kumar. The size of bodies is larger than a possible bacteria. It may be yeast, but it should be checked by microbiological techniques. Some small possible budding parts is seen.
Best regards.
Thank you very much for your answers and suggestions, I appreciate very much. This is a dressing that has been in contact with a wound from a skin graft donor site. I expected to see a bacterial biofilm, but the contact with the wound was very short. You're right, it looks more like microbe. I have a PhD in physics, so I'm a bit far from microbiology.
I'll think about all this!
Thank you again and spend a beautiful day!
Best regards,
Monica
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