What's the difference between 0.1% triton-X100 and ice-cold methanol in the immunofluorescence assay for intracellular protein staining?

Hi, Guys, I noticed that researchers usually permeabilized the cells with 0.1% Triton-X100 or ice-cold methanol (90%) for an intracellular protein staining, it looks like that the two methods work...

15 February 2021 5,606 4 View

How to wash the LiF left by Ti3AlC2 etching?

I added 1 g Ti3AlC2 to 1g LiF and 20mL 9 M HCl and stirred it at 450 rpm at room temperature for 48 h.After deionized water washing and ultrasonic treatment, the product was obtained, but its XRD...

08 October 2020 7,612 4 View

What statistical tests can I perform on glycomics data collected from disease cohort across 3 timepoints?

I have a dataset comprising clinical characteristics and glycomics profiles of disease samples (n=100+) and matched healthy controls. So far, I have been able to do classification modeling to...

20 July 2020 2,153 3 View

How to get qualitative data from a workshop....without a workshop?

I had hoped to run a workshop to understand how militaries and humanitarians should share clinical best practice (or indeed IF they should). It was intended to be a workshop over a day or two - a...

01 July 2020 867 2 View

Where can I submit a letter to the editor or correspondence article?

I am a medical school student in China, and I have written several short articles like 400-600 words regarding Chinese medical policy, medical education and so on. I focus on the violence against...

17 March 2020 5,527 3 View

Dissertation title for Msc in Cybersecurity and management?

I am looking for idea of doing dissertation in cybersecurity and management for Msc. Can someone help me to give the advice on dissertation title and sector and field to research ? Thank you.

25 November 2019 7,657 7 View

Where can I purchase syndiotactic polypropylene (sPP)?

Hello, I'm looking to purchase syndiotactic polypropylene (sPP). Most articles I find cite purchasing it from Sigma Aldrich, but they sold their last stock in 2013. MilliporeSigma has isotactic...

10 June 2019 6,852 6 View

Is there any study or research paper that deals with the disturbances faced by insects in the natural conditions?

There are countless research papers dealing with the menace and destructive nature of insects. In a way, insects are unfortunately deemed as detrimental organisms of the planet as there are...

23 July 2018 2,305 2 View

Is there a standard protocol to look for the parts of insects in the faecal matter of insectivorous birds?

The reliance of birds over insects for nutrition is an interesting ecological phenomenon. The reliance varies from being obligatory to facultative, but the major chunk of bird population are some...

25 June 2018 6,219 3 View

Which type of spectrotometry method is apt for analysing the pesticide trace contents in insects?

Rapid usage of pesticides in agriculture has gained its way into the body of insects. I intend to check the content present from my personal collection of insects like grasshoppers and beetles...

06 March 2018 1,705 7 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View