Insect Lipase Activity?

Hi everyone! My name is Nacho. I am trying to assay lipase activity from insect midgut supernatant, and I have some problems and some doubts about it. I do not have the pH-stat. Thus, the...

22 November 2020 5,595 3 View

Is Tetrabutylammonium hydroxide Mass Spectrometry compatible?

Hello forum, I would like to know if TBA.OH is volatile in mixtures of acetonitrile or methanol. I want to analyze peptides that were previously dissolved in TBA.OH-conatining solution, but dried...

05 October 2020 6,936 3 View

Any good manual for phenomenological approach to sustainability?

I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...

06 July 2020 9,618 1 View

Can RL agent's decision be explained after inference?

I would like to know if there is a method, or sets of method that can explain why a RL agent has chosen certain decision, after the agent has been trained. I'm not looking for different RL...

23 April 2020 151 3 View

Can you desalt peptide digests with high pH RPLC?

Hello! I am working with some materials that require desalting of the peptide samples prior testing. My samples contain a salt of potassium. With a standard sample, I would use R3 or C18 resins...

19 January 2020 6,772 3 View

Methods available to calculate animal density by means of camera trapping?

We have two large camera trap data sets (tropical and temperate forest), which I would like to use for estimating animal density. Which are the available methods? Strengths and weaknesses? Thanks

16 January 2019 5,879 3 View

I have to remove RNA from DNA samples. Using a RNAase A (4 mg/ml), which volumen do you recommend to use?

I have some sample that I have to re-purify and some samples to isolate DNA de novo. In both cases I have to use RNAase to remove RNA. I tried with 1.5 ul of RNAase A (4 mg/ml) but the RNA is...

13 December 2018 860 2 View

Which tail occluder size is appropiate for C57 mice?

We're planning to measure non invasive blood pressure in C57 mice. Weight should be around 20-40 Grams. There are 2 sizes available at Kent Scientiffic: small and extra-small... they're both...

26 June 2018 9,849 1 View

Seminal papers about digital platforms?

25 June 2018 6,246 2 View

How bad is to have frozen cells at a DMSO concentration to DMEM of 2% instead of 10%?

20 April 2018 4,639 3 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View