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17 February 2021 4,217 3 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How do I get spectral power distribution (SPD) of my photoluminescence sample to calculate chromaticity coordinates?

Hi, I have photoluminescence samples which I already characterized and from that I already obtained the steady-state photoluminescence (SSPL) property. I want to draw CIE chromaticity diagram to...

24 January 2021 7,770 1 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

How can one synthesize the Inverted Core-Shell Particles (ICS)?

Dear RG colleagues, how can one synthesize the inverted core-shell particles (ICS) from a polymer and a Nano-metal oxide? Where the polymer is a core and the metal oxide is a shell. Under which...

01 December 2020 9,895 2 View

Why do I get low absorbance in MTT assay?

I have been trying to do MTT assay using HCT-116 cells but I have been getting very low absorbance readings (absorbance is usually 0.25 and below) even though iam seeding 30,000 cell/ well. I have...

16 November 2020 3,588 3 View

What do you think the percentage of educational lost by students in courses where labs and workshops are necessary?

During the current pandemic, most educational institutions all over the world were forced to on-line education which led students to be away from practice applications such as labs and workshops....

30 October 2020 3,254 16 View

What is the relationship (equation) between node transmission range and node density in wsn?

I am simulating routing protocol in wsn the orginal simulation was based on 802.11 mac and physical layer and I am using 802.15.4 as you know the transmission range in 802.15.4 is less, which...

20 August 2020 8,888 2 View

Are you interested in IEEE & Scopus Indexed Conference ?

Dear Colleagues, I am pleased to announce the 3ICT 2020 conference where you can publish your research finding without travelling. All papers registered and presented at 3ICT2020 conference will...

23 July 2020 4,077 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

14 February 2021 3,017 3 View