What's the best fluorophore to use for KI67 analysis?

I would like to check proliferation of human CD8 cells (isolated from blood ) after few days of culture. I have a panel of antibodies and I want to add KI67 to analyse proliferation. Does anyone...

08 October 2020 4,763 3 View

Using proc Glimmix in SAS to fit a generalized logit model, how can I allow for correlations between the random intercepts for various outcome groups?

I am using proc glimmix in SAS to fit a multilevel model for a multinomial outcome with unordered response categories. Proc glimmix requires that you specify the group= option in the random...

12 March 2015 9,536 7 View

How many cells are needed for detection of 2-hydroxyethidium by HPLC-MS ?

I am preparing experiments to determine the superoxide content of cells (CHO and red blood cells) using hydroethidine and HPLC-MS (as described by Zielonka et al, Free radical in Biology and...

02 March 2015 2,066 4 View

Does anybody know the fluorescence duration of DCFDA and DHE ?

How long does the fluorescence of DCFDA and DHE last after fixation and mounting of the cells?

10 November 2013 9,232 2 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

What statistical test do I use when analysing data from the social physique anxiety scale?

I am researching the effect of social media on social physique anxiety of young adults. What statistical test do I use when analysing data from the social physique anxiety scale? (Leary, 2013)

03 February 2021 2,618 5 View

Can I use video interview excerpts from others in public domain (YouTube, Vimeo, etc) for my empirical analysis?

Dear community, I am currently writing my master's dissertation. As a part of the method, I have to include views (interview) of experts for the empirical analysis. The problem is I have not...

31 January 2021 3,438 3 View

Having the moderator continuous or dichotomous? Using MANOVA or the Process macro (Hayes)?

Hi community! Currently finalising my Master thesis research. I manipulated my independent variable to have three conditions. Originally, I proposed a 3(3 conditions of independent v)x2...

30 January 2021 7,821 6 View

Are there existing studies in economic sociology that have applied Kalyan Sanyal's theoretical framework as a methodological basis?

To be precise, I am looking for studies on informal labour that have taken on board Sanyal's category of the Need Economy and his 'Politics of Exclusion' in studying the political-economy of...

26 January 2021 4,219 3 View

Does anybody know about literature on protest and post-conflict processes/societies? What would you recommend?

I am trying to start a project on the subject and your help would be appreciated

18 January 2021 2,190 6 View

No title

03 January 2021 3,227 1 View

How the year 2021 can be made good for global community?

Many steps can be taken to make the year 2021 good for humanity. For instance, if powerful and rich countries decide and spend some part of their resources for the improvement and betterment of...

30 December 2020 830 4 View