Hello, if the plant extract contains steroids based on its phytochemical results, is steroid an antihyperglycemic agent?

Our plant sample contains steroids based on the phytochemical results, and were having a hard time looking for some references about steroids' effect on lowering blood glucose level on mice.

31 January 2020 9,763 3 View

How to mutate a DNA sequence to stop binding to its specific primer?

I am doing reverse transcription using a semi-suppressive PCR therefore I introduce a template switching oligo at the 5' end of my transcript which is complementary to the PCR handle at the 3'...

03 October 2018 4,282 2 View

Is anyone aware of a plugin out there to perform discriminant analysis in PSPP?

If so, could you point me in the right direction? Can't seem to find anything.

09 January 2014 3,600 1 View

Need help making a Fiji (ImageJ) Tool

I'm working on manually analyzing a series of photographs of archaeological artifacts. Each photo contains a series of stone tool fragments laid out on a neutral background. I'm using Fiji image...

06 January 2014 5,335 10 View

Any common protocol for inflammatory stimulation of murine endothelial cells in vitro?

26 December 2013 1,701 9 View

Can the measurement of cytokine levels according to their half lives be useful?

A question of cytokine kinetics. In an activation assay, we usually want to measure effects of our treatment (whatever this might be) on pro- or anti-inflammatory cytokines. Most commonly...

28 November 2013 2,096 3 View

MPO activity assay - can anyone help?

I homogenized murine lungs in 0.5% w/v HDAB in 50mM sodium phosphate buffer to perform a MPO activity assay. The protocol I do have instructs that I should use 20ul of my samples with 160ul TMB...

01 October 2013 8,409 3 View

How fast should an ELISA plate be read?

I finished an ELISA assay and read it to discover two hours later that I saved the 630nm data which I used to correct my 450nm data. I immediately measured the plate again but I'm not sure whether...

05 September 2013 4,109 21 View

qPCR data representation

I have some qPCR data which I want to represent in graphs. I used to use the DCt values but the problem is that they are reciprocal to the expression changes (high DCt values means low expression)...

13 July 2013 4,875 2 View

What are your experiences with conducting (and publishing) online surveys?

I am interested in conducting an online survey and curious as to the requisite ethical approval and the receptiveness of peer-reviewed journals to the results. If anyone has any experience in...

02 July 2013 8,531 40 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

No title

01 March 2021 3,905 5 View