Hi, I am not sure what material you are studying. However, you can use flowers e.g. carnation cut flower an ethylene sensitive flower as a test material to see inhibition of ethylene biosynthesis with your chemical. You can study in two ways. You can use your chemical as continuous treatment or as pulse treatment (little higher concentration and short time treatment 10minutes). Continues treatment means putting the cut flower in x concentration of your chemical until senescence and pulse treatment means treating with x concentration of chemical and for x time and putting in distilled water until senescence.

1. You can use various concentration of your chemical plus distilled water control and Silverthiosulphate (STS) 0.2mM (pulse treatment of 10 min) as positive control in a vase.

2. Keep these flowers in a climate controlled environment or in well ventilated room.

2. Flowers will be observed daily and if the flowers treated with your chemical last more than control, it may indicate inhibiting ethylene biosynthesis.

3. Further, the sign of ethylene inhibition with your chemical treated flower is no in-rolling of petals as petals will in-roll in control (distilled water only). No in-rolling of petals with STS treated carnation flowers.

4. You may measure ethylene with gas chromatograph from the chemically tested cut flowers and control to assess quatitatively the inhibition of ethylene biosynthesis. Low or no ethylene detection in your chemical treated flowers will indicate inhibition of ethylene biosynthesis with your chemical.

5. If you want to know how your chemical is inhibiting ethylene biosynthesis, you may need to do further study. Two enzymes are involved in ethylene biosynthesis ACC synthase and ACC oxidase.

Dear Umed, thanks a lot for your detailed answer. It is very helpful. I will try it soon.

Best wishes

Zha