We are going to be monitoring GABA levels via microdialysis, and most publications I've read show that GABA is especially sensitive to the aCSF formulation. Can anyone attest to a particular recipe that works well?
You are wise to consider that GABA microdialysis has its complexities. The main issue for brain microdialysis of GABA and glutamate is that both of these neurotransmitters have a substantial extrasynaptic pool, which is thought to contribute to the majority of the signal. Evidence for this would be that application of TTX does not abolish the signal, in fact only minimally attenuating the signal in some cases. If your intent is to sample synaptic GABA, then you may have your work cut out for you. Some labs use aCSF that has a higher calcium concentration to promote sampling from the synaptic pool, but this really depends on your experimental question.
There are several articles addressing this issue. Here is just one: Article Sampling glutamate and GABA with microdialysis: Suggestions ...
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