That´s the question :)
Regarding CAR-T cell therapy, during co-cultures with different effector:target ratios, when we refer to "effector", does it involve the total T cells? For example, does a ratio of 5:1 mean 5...
28 May 2024 2,757 4 View
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04 March 2024 3,344 3 View
Hi! I was wondering if anyone has taken confocal images of apoptotic cells stained with Apotracker Tetra, from Biolegend. There is information using Apotracker Green, but I haven´t found a...
06 February 2024 8,343 0 View
Hello, has someone performed a T cell FACS Sorting after lentiviral transduction? We would like to purify those T cells that were transduced using GFP as reporter, and continue expanding the cells...
21 January 2024 2,541 0 View
Estoy interesado en informarme acerca de la relación entre la neuroarquitectura y la música. Digamos, efectos de la música en el diseño edilicio o arquitectónico, efectos en de las edificaciones y...
18 October 2023 2,789 0 View
Hi! We are producing lentivirus for T cell transduction. However, there are several protocols that do not mention the use of polybrene for titration in HEK293T, but they use centrifugation...
03 October 2023 7,363 2 View
Dear community, I want to ask you a methodological/statistical question. We performed ten independent hierarchical linear regressions divided by gender for an article writing (N=1502...
12 September 2023 2,386 2 View
Hi! I want to induce an in vitro exhaustion model for human T cells, purified by magnetic columns. I found a protocol in which the authors cultivate the cells with Dyna beads (I have other...
15 August 2023 6,577 0 View
How are relations about spirituality ,religion and mental health?
12 August 2023 6,407 2 View
I need calculate RMSD between ligands
04 May 2023 4,961 3 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
need to open an account to upload my published papers
01 August 2024 9,255 1 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
When the eyes of a person are damaged this causes complete blindness. Likewise, when Wernicke’s and Broca’s areas of neocortex are damaged this causes complete aphasia, losing the ability to...
01 August 2024 6,744 2 View
