Are there any methods to detect vitamin C in tissues or cell extracts?

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Robin Joshi

Yes, there are a lot of methods to analyse Vitamin C. You can go with HPLC with 0.1%H3PO4 as a mobile phase.

gudluck

Eshan Sharma

try this protocol.... works well in our lab.. Foyer CH, Rowell J, Walker D (1983) Measurement of the ascorbate content of spinach leaf protoplasts and chloroplasts during illumination. Planta 157:239–244

http://www.springerlink.com/content/t623l285231pt278/

Hi mark,

The protocol works well with protoplasts from plant cells also. so in general for " tissue or cell extracts " its would not be bad to give it a try as its pretty simple one.