Good day. I have difficulty applying my data set to a Machine Learning Clustering algorithm, can anyone assist me in this regard?

I have a data set for a railway wagon: It consists of 9 measurement parameters per wheel (hollow wear, flange thickness, etc) and there are 8 wheels on a wagon. I need to work out a health index...

23 June 2020 838 11 View

How do I change category from an article to a book?

Several books have been listed as articles.

16 April 2020 6,631 3 View

How to specify accurately the Parameters for the slope and intercept when using Latent Growth Modelling?

The usual procedure when using a Latent Growth Modeling with, for instance, 4 time points, is to code the intercept T1=1, T2=1, T3=1, T4=1 and the slope T1=0, T2=1, T3=2, T4=3. From my...

14 February 2020 7,172 6 View

The astigmatism of the eye ==> some quantic problems. Ideas and experiments ?

The astigmatism of the eye ==> some quantic problems. No one photon can be 5 m wide on the mirror of the Mount Palomar, simply because the geometrical defects of the mirrors limit the size of...

29 January 2018 1,475 4 View

Does soil fertilization impact differently on shoot growth in trees and on the number of leaves on these shoots?

Is it possible that fertilization of trees by spreading N-P-K on soil brings to a longer annual shoot growth but in an other hand they will have less leaves each?

25 November 2017 9,852 22 View

Do you know the locality of "Ain Mauddud" in Qatar?

The 1975 lexicon of Qatar states that the Cretaceous Mauddud Formation got its name from that locality near Dukhan.The name was assigned by Dr. F.R.S. Henson in an unpublished 1940 report. Does...

30 July 2016 6,048 6 View

What is the best marker of human kupffer cells in immunohistochemistry?

I'd like to make an immunohistochemistry on human samples and find the kupffer cells. How can I do that? Thank you!

17 January 2016 7,574 3 View

Anyone familiar with rearing locusts (Schistocerca gregaria) in great quantity?

06 February 2015 6,753 4 View

What are the assumption of equal spacing of the repeated factor in repeated-measure ANOVA?

In repeated measure-analysis of variance (univariate, multivariate or mixed models), when time is the repeated factor, do times have to be equally-spaced? Any citable reference would also be...

13 August 2013 9,051 1 View

Looking for validated qPCR primers for estrogen receptors (esr 1 and esr2) in rat

In order to determine the effects of endocrine perturbators in RAT cells from colon tumors, I want to determine their impact on mRNA expression of esr 1 and 2 . Due to the low level of esr 1...

30 October 2012 2,743 7 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Examples of GAMS syntax for a permutation flowshop scheduling problem?

Hi everyone, I am new to the field of industrial engineering. I need to formulate a gams model for a permutation flowshop scheduling problem. I will appreciate if someone can send me a code...

09 February 2021 891 2 View