Hi, I would like to know if any has some insights about colonial stage conservation of P. pouchetii at long term. I stored seawater samples with lugol at 4C for above a year. I was wondering if the colonies could have been disrupted over time. Thank you!
Got my answer:Rousseau et al. 1990 showed that a mix of gluta, lugol and iodine can keep the colonial form of the Phaeocystis. Thanks!
Well, independent of the method of preservation, the morphology can change.
I would be pretty certain that the integrity of the colonies are not preserved, certainly not over that time. Even if "Psedulogols" are used as this mixture of glutaraldehyde and lugs are often called. Instead the best you can do is live samples in a FlowCam, or put them on a filter and freeze (Estep et al)
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