Hi dear Frendz, I need full methodology for extraction and measurement of APR activity in plant leaf, stem and root .. I have got some information like one i had cited below.... but i need full research articles for complete methodology of APR.
APR Enzyme Measurement
Plants were washed for 1 min with H2O at 4°C, and extracts were prepared by grinding 1:10 (w/v) in 0.1 m Tris-HCl, pH 8.0, containing 100 mm KCl, 20 mm MgCl2, and 10 mm dithioerythritol in a glass homogenizer. The homogenate was centrifuged for 10 min at 10,000g and the supernatant was used for the enzyme assays. APR activity in extracts was measured as the production of [35S]sulfite, assayed as acid volatile radioactivity, formed from [35S ] APS in the presence of dithioerythritol (Brunold and Suter, 1990).
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