Anyone with a good reference for carrying out quantitative microbial risk assessment for Salmonella and E.coli from food using quantitative PCR?

More Cianjoka Gichuyia-Kibui's questions See All

Protocol for extraction of salmonella and e.coli bacterial cells from enrichment? I would like to store the cells as pellets at -80 too, any advice?

I would like to carry out qPCR for quantification of Salmonella and E.coli from pork samples and vegetable salads. I would like to extract and store the bacterial cells first as pellets before...

06 March 2019 9,578 3 View

Protocol for extraction of salmonella and e.coli bacterial cells from enrichment? I would like to store the cells as pellets at -80 too, any advice?

I would like to carry out qPCR for quantification of Salmonella and E.coli from pork samples and vegetable salads. I would like to extract and store the bacterial cells first as pellets before...

02 March 2019 3,990 3 View

What are safe ways to connect to the PostgrQSL Database from Web Maps?

I want to use PHP to pull data from the PostgreSQL Database into my application. I wanted to know whether this method is secure, if my various users will have to interact with data.

03 December 2014 1,242 4 View

GIS Water-Demand visualization app, which is the best way to develop a "water-demand" visualization app?

Which is the best way to develop a "water-demand" visualization app where a company can have real time data updates from a master meter into the systems in offices.

26 October 2014 9,635 4 View

What are the best (less toxic to human) methods of microbial lab fumigation?

We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.

04 August 2024 1,973 1 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

What is climate resilience for food security and sustainable agriculture and how is smart irrigation in agriculture climate resilient?

22 July 2024 1,947 0 View

Why do fish have fishy odors naturally ?

Chemical Engineering /Food Engineering

20 July 2024 4,152 4 View

How can sustainable agriculture practices mitigate climate change and ensure food security and role of agriculture in environmental sustainability?

17 July 2024 1,712 12 View

Does the bread-making process lead to a sufficient reduction in lectin activity of bean flour to guarantee food safety?

I'm currently working on the implementation of pulses (legumes) in bakery applications. In one case, pulse flours could be added to bread to increase the nutritional value. Pulses (in particular...

17 July 2024 7,293 3 View

How can technology contribute to global food security and sustainable agriculture practices contribute to poverty reduction and food security?

17 July 2024 7,745 6 View

Do software tools exist to assess the economic and technical practicality of introducing new food products, such as yogurt with modified starch?

This question explores the world of food innovation and asks if there are computer programs that can analyze the financial and technical feasibility of introducing new food products. For instance,...

13 July 2024 7,446 0 View