Anyone know how to make the calcs for determination malondialdehyde by UV-VIS SPECTROPHOTOMETRY?

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Can a semi-conductor material have different absorption coefficient?

Does the absorption coefficient of the semiconductor changes with the change in the structure of the semiconductor? If you have a thin film and nanowire of the same semiconductor, will the...

23 February 2021 9,799 4 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

absorption= 1-mag(S(FP1:1,FP1:1)S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)S(FP2:1,FP1:1)) is it correct for absorption ?

hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...

17 February 2021 9,178 2 View

Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

How to detect uv vis spectrum of nanoparticle under 300nm?

I try to make the indium nanoparticle and check the uv vis spectrum. I heard that In nanoparticle has surface plasmon resonance peak at 200~300nm. But most of solvent has absorption peak under...

14 February 2021 6,950 15 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

Bruce Neagle

What method are you using? As I recall the reagent (as listed by APHA or AOAC) was TBA or Thiobarbituric Acid. Why do you want the extinction coefficient?

Jean Rene Grezes

Your question is a little obscure! Determination of malondialdehyde IN WHAT???

Please reformulate your question if you want to do a colormetric assay according to:

https://www.nwlifescience.com/nwa/product/malondialdehyde-mda-assay-kit-200-tests

Citation from above link.

A colormetric assay kit for detection of Malondialdehyde (MDA) or Thiobarbituric Acid Reactive Substances (TBARS) in multiple species and sample types. Designed for simplicity and affordability, this assay utilizes various improvements to provide the most dependable data among commercial assays of this type.

I agree fully with the answer and question posted from Bruce:

What do you want to measure with the extinction coefficient? (ipse dixit Bruce)

JRG

here a link of the above link concerning the calculation (see page 7):

Derivative specroscopy is used.

https://www.nwlifescience.com/product_insert/nwk-mda01_derivative_spectroscopy_product_insert.pdf