How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View

How do you interpret and understand 11B 3Q MAS NMR graph?

I am a novice to NMR and need help in understanding what i am looking at in terms of 11B 3Q MAS NMR graphs. Any resources for beginners would be helpful in interpreting the data. Literature only...

20 January 2021 2,648 3 View

How to prove the formation of a synthesis product using NMR ?

Hello, I am trying to identify the formation of a synthesis product. Knowing the precursors, I focused on the identification of the H-C long-range coupling between sites (a/b) and (a/c) using...

19 January 2021 8,438 5 View

Can Cryo-Sem be used for small protein 3D structure building?

Hello, I have a small purified protein, 15kDa. Iam seeking some technical service to build its 3D structure by Cryo -Sem, or NMR. However, I was told by some technicians that Cryo-Sem is only...

12 January 2021 5,072 3 View

In which laboratory of India 13C NMRanalysis of solid samples could be performed?

I need to analyse some solid samples (soils) for 13C NMR. Is there anyone who can help me out? The contribution of the contributor would be properly credited during any sorts of publication....

07 January 2021 2,016 4 View

Could anyone suggest the appropriate composition of ball milling spheres for coal ash treatment?

I have built a ball milling to use mainly on the fragmentation of coal ash. Now I need to acquire the spheres to operate it. Steel spheres are cheap, but I am afraid they can liberate iron as it...

06 January 2021 6,433 2 View

What is the use of Carbon and Proton NMR Spectrum in Peptide Analysis?

Am in Peptide / Protein Research, may i know what useful analytical information we are getting from 13C & 1H NMR spectroscopy analysis? Somebody getting NMR for smaller proteins, any useful...

05 January 2021 1,196 7 View

What are the minimum amount of polymer samples needed for 1H-NMR and 13C-NMR?

I'm working on polymer. I've tried 8mg sample in 0.6 ml solvent for 13C NMR but I got a high noise spectrum. Any specific amount for polymer sample in running NMR ?

20 December 2020 506 4 View