I am not sure genital organs will be fully developed inside pupa (may be except the very late stages). Moreover, genital organs might be soft and therefore deformed and not suitable for treating taxa.

For pupae that didn't manage to hatch but were fully developed, I made genitalia preparations without any problems following standard procedures. They were not less sclerotised than those from an imago and could not be distinguished from the latter. But surely, the pupa must have reached the stadium shortly before hatching to have the organs fully developed.

My experience is similar to that of Theo.

Its very difficult, but why from pupae??

It is not more difficult than making preparations from a hatched adult and sometimes it is very convenient if one can make a genitalia preparation from a pupa. For instance: a pupa died shortly before emergence and you want to confirm (or investigate) which species it is.

I'm sorry for very late reply.

Yes, Sir Theo Garrevoet & M.J.W. Cock . I have done the work, and indeed the pupa can't be analyzed by genital organs. Genitalia can be observed during the adult phase (butterfly). Thank you for sharing your knowledge, Sir 🙏

And thank you for your paper, Mr M.J.W. Cock . I learned a lot from the reviews you wrote :)

That's right, Sir Faisal Jaber & Dmitry Telnov . Because I'm curious, it can be observed that the genital organs are in the pupal phase. And apparently no.

Thank you Santosh Singh Santosh Singh , I did that stage exactly as you described ^^

Indeed, from a freshly pupated specimen one can not investigate the genital organs because they haven't formed yet. But shortly before the imago hatches these organs are completely developed and can be studied without any problems.

Thank you for the knowledge, Sir Theo Garrevoet and Sir Santosh Singh. I strongly agree with what you're say, Sir. I'll try to make genitalia preparations from imago, to confirm this problem.

Thank you..