A plant material which its leaf is used in local remedies by adding in a boiled water and inhalation was considered. The leaf have been collected and dried in shadow at room temperature. The dried leaf was then milled using analytical miller. When the sample was in the hydro-distillation process my research colleague and me were getting headache. This situation was happened 2times in different days.
Are you using open flame for heat?, or electric heating mantel? If open flame you may be creating carbon monoxide. If heating mantel- is it clean, could someone that used it previously spilled or over boiled a chemical on it, that is off gassing?
Are you using organic solvents?
Are you performing experiment in a fume hood? If not set experiment up in fume hood and see if that helps.
Peter Philbrook
we were using heating mantle and there no organic solvent (only water) used in the activity. OK, i will try to use the fume hood. Thank you for your response.
But, will such headache happening indicate the presence of biologically active compound?
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