To be more specific, we detect luminescence of cells to measure luciferase activity. Thanks.
The resistance reading from WPI EVOM2 is ohm x cm2 or just ohm? I read the manual very carefully and thought it's ohm x cm2 and we don't need to convert it. But the TER value I got for my RPE...
05 June 2015 5,231 2 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...
13 June 2024 9,936 5 View
Hi, I've recently started using the FLUOstar Omega plate reader to perform some basic bacterial growth curves. Before putting the plate in I've been trying to use the temperature incubation...
06 June 2024 5,923 0 View
I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...
02 June 2024 7,833 2 View
Good day! I am currently testing my sample for MIC, may you help me how to interpret the results at OD630? how would I know if there is growth or not based on the values given by the microplate...
01 June 2024 7,920 3 View
I want to use Fisherbrand 96 well plate for my plate reader, but the machine does not have fisherbrand, so I have to find it manuallly. Also the plate is clear but in the machine we have costar...
30 May 2024 2,779 0 View
I'm working on a project related to Luminescence Properties of Rare Earth Glasses (not Europium). I have read some articles related to Judd Ofelt Parameters, but I couldn't able to understand the...
26 March 2024 2,287 0 View
My transfer buffer Ph is 8.7 but should be a 8.3 is this causing my exposures to be off also my bands look smeared. i am trying to troubleshoot and figure out why my chemiluminescence western blot...
12 March 2024 7,433 1 View
Hello Performed experiment nucleic acid extraction by Phenol: chloroform; isoamyl alcohol method, 96 wells microplate spectrophotometer further done to study quantification with260nm/280nm.....
14 February 2024 860 4 View
Hi, I am interested in performing a reporter assay in organoids grown in matrigel. Is there a commercially available kit that would allow me to read both firefly and renilla luciferase...
06 February 2024 6,029 0 View