Anyone has any idea to avoid microplate reader position bias?

07 July 2017 0 9K Report

To be more specific, we detect luminescence of cells to measure luciferase activity. Thanks.

Badges
Science topic

More Christine Cuiping Zhao's questions See All

Any advice on TER measurement with WPI EVOM2?

The resistance reading from WPI EVOM2 is ohm x cm2 or just ohm? I read the manual very carefully and thought it's ohm x cm2 and we don't need to convert it. But the TER value I got for my RPE...

05 June 2015 4,975 2 View

Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

Can someone explain why my A260/A280 ratio is lower than 1?

We are conducting a measurement of Ribonucleic acid from baker's yeast (S. cerevisiae) (Sigma-Aldrich R6750) concentration using Eon Biotek Microplate reader. We dilute the RNA in various...

24 January 2021 9,830 3 View

Should I normalise data when measuring fluorescence intensity ?

I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...

03 January 2021 5,554 5 View

.In my results the slope of curves got from Microplate reader is seems less (less than 1) and negetive some times. What is the reason for that?

In my research I'm enumeraring the florescence intensity (FI) Vs E-coli concentration in river water. I measure FI by Tecan I control 200 pro and E-coli by Membrane filtration.

03 January 2021 2,991 3 View

Is it better to normalize Nile red absorbance to Bradford assay or to cell number?

I stained the fat droplet inside the cell using Nile red and read the absorbance by a microplate reader. Which better to do, normalize the Nile red absorbance to Bradford assay or to cell...

12 December 2020 8,036 2 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View

Assay with absorbance/fluorescence readout?

I'm setting up a liquid handling robot and need a simple assay to test it. Ideally I would like to mix 3-4 reagents and have a absorbance/fluorescence readout on a plate reader. It's also possible...

11 November 2020 7,645 3 View

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

30 October 2020 1,513 2 View

How I can find the quantum efffcincy from the measured and calculated decay time when the measured decay time is greater than the calcualted decay ?

I am working on the luminescence properties of Nd3+ ions doped phosphate glasses for which I have to study the decay time. I measured the decay time experimentally and also calculated the decay...

16 October 2020 951 7 View