Hmm, maybe I should qualify my question since it seems to have gone off track. I was talking about testing the ability of your sanitizing solutions in reducing bioburden in a controlled study. I was wondering how often organizations repeated this testing since I need to come up with a protocol for my company.

We used to test newly delivered lots of disinfectants for performance qualification. There are existing protocols you can follow for that. :)

Ugh...that would be a lot of work if we had to do that every time we got a new lot in. For one test, it takes a full week to perform including weekends. I'm hoping to just do an annual requalification of solutions and in-house isolates. Out of curiosity though, do you have a link to any of those protocols?

I do not have access to the company protocols from where I used to work before but I searched a little over the net and found some stuffs which I thought might be useful for you.

http://antimicrobialtestlaboratories.com/

http://www.astm.org/Standards/E2614.htm

http://www.ats-labs.com/services/disinfectant_test.htm

http://www.nelsonlabs.com/TestingServices/Tests/disinfectant-tests.aspx

What I remember was that we follow the USP method for testing our disinfectants in the lab (Microbial Limits Test)

http://www.microrao.com/micronotes/pg/testing_of_disinfectants.pdf

Best regards,

Kris

Much thanks, I'll take a look! :)

Good luck Jeremy! :) Tell me if you've made progress on it.

Yes, we have done several of these studies for companies and as part of Environmental Public Health Research and Outreach Activities.

Yes we do environmental microbial study regularly (monthly) of the ot, icu, iccu, nicu, labour rom and the opds also. If there is any civil work done in ot's we test for anaerobes and fungi also.routinely we look for aerobic bacterial concentration, we have done few studies related to this like nasal carriage rate of staph, fingers and webs for staph and enterococci, stethoscope , cellphone swab cultures for microbial flora. We presented a poster at mumbai

The use of antiseptics and disinfectants such as phenols and Formaldehaadwo Dettol

we are also doing environmental studies regularly but we test for anaerobes in ot and nicu, and icu. In case of outbreak we test for aerobes. Is there any standard protocol for this?

Sterilization using chemicals or radiation

Hmm, maybe I should qualify my question since it seems to have gone off track. I was talking about testing the ability of your sanitizing solutions in reducing bioburden in a controlled study. I was wondering how often organizations repeated this testing since I need to come up with a protocol for my company.

Sterile surface dry heat kills bacteria capsule because of clotting proteins and moist heat more efficiently

Used formalidehyde

On our organization, specifically on microbiology area each month we apply sanitization probes why? because is imperative demonstrate that the working area is clean & aseptic. The best form to assure the effectiveness of sanitization products is rotate the use of variuous products. For example etilic alcohol, fenol, sodium hipochlorite; each twice weeks change the product that we use and each month we apply a challenge test to the 3 products in this challenge we take a sample of the area (by contact of the surface), second step apply the disinfectant, third step take a sample of the clean area, forth step put the hisop on nutritive broth, fifth step take an alicuote of nutritive broth and put on petri dish with a media like that nutritive agar or similar. If the sanitization product is appropiate for the purposes you can do to use. On spanish you can view a similar protocol on goverment web site, if you need the link tell me

Yes

This process testing should be on a repeat basis every day!!!! within the Vaccine sterile drug manufacture industry

if you require any help please contact me

reg's Dean

Hello I mean in this research the number of cells or the amount of bacteria and the type of bacteria on the one cockroache. And record the number of resistant bacteria from antibiotics ask for any search or article for this topic if possible and thank you

Hello Amany

I Have not required any information as to a cockoache study really? As far as medical human vaccines within the bacteria arena been resistant bacteria to vaccines , there are 1000,'s and depending on what type of roach and cells you are looking at really ! I wish you well in your research

i believe that you are pertaining to a process validation experiment. well, you have to do it frequently for a defined period of time until you get sufficient data (say do it weekly or bi-monthly over a period of one year or six months) and do statistical process control analysis/techniques, see if your data is within control for that particular time frame, then gradually decrease the frequencies of your tests over time (say, on a quarterly basis or a bi-annual basis) if it is within control you also have to write specific action plans whenever your values approach alert/action limit status. I believe that it is specified in several clauses in an ISO document for bioburden and contamination control.

Hello Kris

Thank you for your email, In regards to your comment I have to do control studies on every aplication per day as with over 1500 products and two types of material and over 500+ clients I wish I could just report data per month or qtr but ethical standards and client request stop me in dong so,

Sorry didnt get to finish that convo with you its 330am in australia and its been a long day But yeah as I was trying to say it does make it hard to have required client data when requested no just owaa did it last month and it helps with the TGA audits

Do you produce vaccinesby any chanch as I havent met anyone on this web that does just a thought ?

Vaccine Vials like this

Manufacture commercial qtys

Thank you

Currently do not use your idea to the other time I use the type of bacterial resistance to various vaccines. Currently used antibiotics and study the resistance of bacteria isolated from cockroaches to many antibiotics

Thank you for info.