Where can I find these two papers?

14 January 2021 1,572 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

Is there any ingredient I an add into formula to slow down gas generation from Vitamin C?

I know Vitamin C is unstable and tends to be oxidized quickly. The problem here is I made a Vitamin C beverage at pH 4-5, and it starts to generate gas in the packaging, especially at high...

13 December 2020 6,055 1 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View

Why case-controlled study is more bias prone than cohort study?

I noticed from several articles saying that case-controlled study is more bias prone than cohort study (in the context of cancer early detection using molecular marker). I spent sometime to try to...

17 November 2020 1,878 4 View

How to perform statistic analysis for age range between two cohorts?

Hi, I was reading the paper (MicroRNA Expression, Survival, and Response to Interferon in... ). In Table 1, I'm wondering how did the authors calculate a p-value of 0.29 for Age? The age in Table...

20 October 2020 2,276 5 View

Is RNALater (Qiagen) or Nucleoprotect RNA (Macherey-Nagel) compatible with High Pure Viral Nucleic Acid Extraction Kit?

I am planning to preserve fresh bat guano in either RNALater or Nucleoprotect RNA but my lab has an excess of High Pure Viral Nucleic Acid Extraction Kits. Any idea if both reagents are compatible...

14 October 2020 2,848 2 View

Hi Dear, Could someone figure the pH out?

There are two Triz-base solutions, the pH of solution #1 is 6.85, the pH of solution #2 is 9.97. What is the pH after mixing the same volume (50 ml) solution together. Please figure it out step...

29 September 2020 1,741 6 View

Is standard deviation of residuals of Markov switching GARCH model regime specific?

I'm using MSGARCH package in R. By return_data/Volatility(fit.model), I get the residuals. When I calculate the standard deviation of the residuals, it turns out that it's close to 1 for all...

14 August 2020 3,132 6 View

Any recommendations on the software to do text mining or sentiment analysis on Chinese reviews?

I am trying to do text mining on Chinese reviews. I have tried out many softwares, like the RapidMiner, Chinese Text Analytics, Python. Most of them seem to require certain level of programming...

12 August 2020 9,109 8 View

What is the classification of tree species by texture analysis technique on drone images?

Hi My goal is to distinguish forest tree species (in dense forests) with drone images. I did not get acceptable results using spectral features. Because the pictures were taken in the fall and the...

07 January 2021 7,494 3 View

How to classify a base object with drone image?

Hello beloveds. I am classifying dense deciduous trees with eCognition software. In fact, it is the separation of tree species with drone images. The image has a near infrared band, red, red...

29 December 2020 5,900 2 View

How create a table of variables importance for Random Trees classification in eCognition?

Hello, I would appreciate if someone could advise how I can get the table where I can see the most important layers for RT classification in eCognition. I have around 20 layers as input...

01 February 2020 5,926 4 View

What are the open source options for object-based image analysis?

I am using UAV data for mapping geomorphological processes in different environments, from coastal and estuarine subtropical areas to subpolar and polar glacial landscapes, and I want to profit...

11 December 2019 5,694 6 View

How to perform an image segmentation using eCognition?

Hi All, Kindly, could anyone indicate the best tutorials to learn image segmentation and object-based classification using eCognition Developer? Thanks.

11 December 2019 4,737 5 View

What are the open source options for object-based image analysis?

I am using UAV data for mapping geomorphological processes in different environments, from coastal and estuarine subtropical areas to subpolar and polar glacial landscapes, and I want to profit...

10 December 2019 6,035 13 View

What is the recommended grid resolution (tiling dimensions) for an orthophoto when exporting from Zephyr and importing into eCognition software?

I need to set the tiling for an orthophoto made on Zephyr. I want to conduct object based image analysis on the mosaics made in eCognition software. I am hoping to classify broad tropical marine...

05 June 2019 9,307 1 View

How to perform object based segment validation with the reference layer in eCognition software ?

I want to know the complete step to perform the validation of segments obtained from the OBIA in e-Cognition or in ArcGIS if possible, of LISS-IV image, i.e., to find out the "goodness of fit",...

30 May 2019 1,040 5 View

How to perform object based segment validation with the reference layer in eCognition software ?

I want to know the complete step to perform the validation of segments obtained from the OBIA in e-Cognition or in ArcGIS if possible, of LISS-IV image, i.e., to find out the "goodness of fit",...

04 May 2019 4,761 4 View

What can be the ruleset to extract landslides features in shadow area using OBIA in eCognition software?

In OBIA to extract landslide features, hillshade was used to removed false positives of shadow area but some true positives were also removed. There are some shadow areas in image containing...

01 February 2019 580 3 View