There is no straight forward calculation. There will be loss during processing and storage, it will depend on encapsulation technology, encapsulation matrix, storage condition , the strain used etc......
Yeah. I understand. Do you know, how to inoculate bacterial culture into water column, like beads? I wish to introduce probionts into aquarium tanks with prebiotics, like starch, alginate.
Materials used for Microencapsulation • Various types of encapsulating materials are used for the process. • Namely: – Alginate – Gellan gum and xanthan gum – k-Carrageenan – Cellulose acetate phthalat – Chitosan – Starch – Pectin – Gelatin – Whey proteins – Chickpea protein
The assortment of any material depends on 1) its capsule forming capability, 2) its strength, 3) its enhancing viability of probiotics, 4) its cheapness, 5) its availability, 6) biocompatibility
Biosynthesis of cyclodexrin glucosyltransferase by immobilized Bacillus amyloliguefaciens in batch and continuous cultures" Abdel Naby, M. A., Reyad, M, Reyad and Ahmed, F, Abdel-Fattah Biochemical Engineering Journal 5(2000) 1-9.