Anybody have a protocol for the identification of Borrelia garinii?

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Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Can I transfer the 3D file from Matlab to Ansys or Abaqus?

I have a 3D output in the figure section of Matlab, does anyone know a way to transfer it to Abaqus or Ansys? Or in what format should I save it so that these analysis software can analyze it?

09 July 2024 9,226 3 View

DNA extraction from clinical sample of Staphylococcus aureus?

Salam everyone, So I am trying to extract DNA from isolates of clinical samples of staphylococcus aureus, I am using a QIAamp UCP kit. I followed the same manufacturer protocol and used Qubit to...

03 July 2024 3,892 1 View

Plant extract sterilization???

Dear researchers, I've been working on aqueous plant extract sample, then I autoclaved them in 10 min to sterilize and added some preservatives. However, some of them showed bacterial and fungal...

11 June 2024 1,040 2 View

Editora Artemis, Editorial Académica Española: Predatory publishers?

Hello, On the basis of the conferences I have attended, I was contacted first by Editorial Académica Española to publish a book with them (a development of a conference paper), so I would like...

09 April 2024 1,751 3 View

How to eliminate a recurring mold contamination in the CO2 incubators?

Dear researchers, We are struggling with neverending mold contamination in all of our CO2 incubators. In the past, we used to do H2O2 decontamination/24hr-UV mode every two months now we do it...

04 January 2024 3,717 4 View

How can we improve the sonication process to achieve homogenous fibrils of the desired length (<= 50 nm) as indicated by Dynamic Light Scattering?

We conducted Dynamic Light Scattering (DLS) before and after sonication of our preformed fibrils of alpha-synuclein (aSYN) using a microtip sonicator. Our protocol was as follows: Amplitude:...

10 December 2023 2,768 1 View

How to statistically analyze species diversity more than two communities?

Dear coluegues, I want to analyze, parkland woody species diversity along agro ecology (low-land, midland & highland). how can I check if there is a significant difference along agro ecology...

06 October 2023 9,369 1 View

What are the problems of separating imprisonment and what are the solutions?

21 September 2023 299 0 View

Can I create a new sort of Research Item?

Hi, I would like to create a new sort of Research Item (namely workshops, some with video links). Is that possible? Or it should be better included in "Presentation"? Thank you, Hana

21 September 2023 8,926 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View