How to get the most data from a paraffin embedded liver biopt (10 mg tissue) by using advanced sequencing techniques?
I have a liver biopt of a HCC patient which I want to screen for the expression of liver carcinoma related proteins and the genetic makeup. It is 10 mg of tissue embedded in paraffin for making microscopic slides. I want to extract proteins, DNA, mRNA for genotyping and expression analysis by HTP sequencing/proteomics. The sample is unique, so I want to be sure that the procedure gives maximum results (data will be linked to MRI, CT scans and extended blood chemistry, which are available). I consider to put all data in a publicly accessable repository, so any hints for financing this operation would also be useful.
Why not do a re-biopsy to get fresh, non-paraffined tissue? Where is Wageningen? NL. If I can help, I can send tissue and CAT-scan within a month (I am a Herpetologist). The money-issue: What are you looking for? HCC is diagnosed by CT/MRI, tissue is not needed at all. So whats the goal? Regards BMS
Dear Bruno,
thanks for your suggestions. Have you checked my publications on this topic?
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