Dear Aloesi Dakuidreketi,
I had formerly a good experience with PDA (potato dextrose agar), PCA (potato carrot agar) or V8 medium. These culture media are very good to Alternaria species sporulation (dyctiospores production) in general at 20 - 25 oC, seven days.
Best results were obtained when fungal colonies of Alternaria solani were grown in V8 medium at 25 oC in the dark with agitation for seven days (see attachment).
Similar request "How to sporulate Phytophtora infestans and Alternaria solani in artificial medium? What is the best medium for isolation of both of them?" sent Yanuar Danaatmadja in ResearchGate discussion (see answer other colleagues)
https://www.researchgate.net/post/how_sporulate_Phytophtora_infestans_and_Alternaria_solani_in_artificial_medium_what_is_the_best_medium_for_isolation_both_of_them
Best regards
Vladimir
Alternaria has no major requirements. This fungus sporulated easily in any culture medium.
Thanks Vladimir for your ideas and thoughts. I've actually got the article that you had posted and some others and had tried out some of the methods which unfortunately resulted in profuse mycelial growth rather than conidia, which then tells me that Alternaria solani is not really easy to cultivate in vitro or in laboratory conditions.
So I think I just need to manipulate media and temperature with lighting conditions?
The media I've tried out are V8-Agar, Sporulation medium, 25% PDA and WA.
Thanks again for your great assistance.
Cheers,
Aloesi
Hi: if you want to get a few sporelation for morphological identification you can make disc from tomato leafs and place it in water agar, place over each leaf disc another disc of Alternaria solani culture grow on PDA , incubated it for 5 day you will get a unique spores for this fungus.
you can use the SNA medium. it is a poor medium and allows the sporulation of alternaria and other fungal genera, it allows you to see the spores chaining . I used it for the strains of alternaria which does not spore on PDA and it gave good results. incubation time 10 to 15 days at a temperature of 25 ° C
use 2 percent water agar technique, you will get sporulation or can use host decoction agar medium for better sporulation.
Article Characterization of Sporulation of Alternaria alternata f. s...
above article highlights more on sporulation of Alternaria.
Thanks
I know it would be so late answered.
However, I set a method that supports maximum sporulation while do not decrease the infection percentage.
I move A. solani to new PDA media (pH 5.5) for five days at 12/12 light/dark and 28 C°.
After that I removed surface mysilium gently and keep the plate (without cover) under black light for 8 hours and 48 dark place.
Finally, I collected sporue by dissolving the plate in water.
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