I am keen to know the most suitable reference gene(s) to normalise the qPCR for understanding the basic biology of mitochondrial OXPHOS complexes.
How do you want to normalize your data? If the gene in question is on the mtDNA genome, then you could normalize to the # of mitochondrial genomes per cell. If the gene is from the nDNA genome, then you'll most likely want to use a nuclear, single copy housekeeping gene.
Also, what organism? If you're using C. elegans, then you can use RNA from a given # of worms worms since we know the exact cell number per individual worm.
How do you want to normalize your data? If the gene in question is on the mtDNA genome, then you could normalize to the # of mitochondrial genomes per cell. If the gene is from the nDNA genome, then you'll most likely want to use a nuclear, single copy housekeeping gene.
Also, what organism? If you're using C. elegans, then you can use RNA from a given # of worms worms since we know the exact cell number per individual worm.
I agree. My gene of interest is nuclear encoded though its uncertain that all housekeeping genes are consistantly expressing in any given scinario. For instance, different KO cell lines of oxphos systems.
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