I am fabricating a microfluidic device in PMMA material substrate. The device will be used for culturing yeast cells, which would require some oxygen supply. From literature, I found that PDMS devices have high oxygen permeability which allows ambient oxygen to diffuse through thin PDMS walls and enter the cell culture chamber. i would like to know, can the same be achieved with a PMMA device (1 mm thick). What is the oxygen permeability rate of PMMA compared to PDMS?
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Please , Look at links here , i hope help you
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http://www.sciencedirect.com/science/article/pii/S0956566314005302
http://www.rsc.org/images/LOC/2011/PDFs/Papers/047_0500.pdf
http://web.mit.edu/biology/sinskey/www/ZhangMicrochemostat06.pdf
Article A reliable method for bonding polydimethylsiloxane (PDMS) to...
The diffusion rate of O2 in PMMA is about three orders of magnitude lower than PDMS but depending on the head space over your cells, the perfusion rate, the cell type, and the type of experiment you plan to run, you may not need to worry much about the gas permeability of the fluidic structure. Your cells will require a constant flow of fresh media to survive more than a few hours. This media will have O2 dissolved it that will be adequate for cellular respiration (assuming it has been exposed to atmosphere at some previous time and the temperature has not been above the experimental temperature). If you need to stop the flow to let the cells adhere to the surface, then you may have some issues. You'll need to consider the respiration rate, number of cells, volume of media, concentration of energy substrates, and dissolved O2, as well as the acidification rate of the media , when attempting to determine the maximum allowable stop flow time for your fluidic design.
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