I have spinal cord cryosections where I would like to check the axonal regeneration after the spinal cord injury. We tried GAP 43 which seems not quite specific for regeneration in our case. Now we would like to try SCG10 and CAP-23 using IHC. I wonder if anyone has tried them before, if so let me know. I also would appreciate other suggestions.
regeneration associated genes and proteins that can be used are:
Gap-43, Cap-23, L1 and N-Cam
trophic support is bad in CNS: thus BDNF and NT-4/5 are hardly to be expected.
if regeneration is expected look for markers of oligodendrocytes, astrocytes and activated microglia.
One of the bets axonal regeneration markers is Neurofilament-M antibody, or Tau-1.
If you need detect sprouting after a spinal cord injury, you need use a peripherin antibody.
in may last paper I labeled the regenerate axons after spinal cord injury
best,
Ramiro
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