16 April 2018 6 3K Report

finding a suitable final year project tile for waste management (construction)

More Jing Wen Yap's questions See All
Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

How to get LP fiber modes directly from Comsol?

Hi there, I'm new to modelling with comsol. I would like to ask if it is possible for comsol to output linearly polarized (LP) modes? I tried modelling a simple single mode fiber with an enlarged...

23 July 2024 4,019 3 View

Mass spectra averaging algorithm?

I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...

22 July 2024 3,975 1 View

How to combat antibiotic resistance?

The increasing resistance of bacteria to antibiotics has made many infections difficult to treat, posing not only a threat to public health but also a major challenge to healthcare systems worldwide.

18 July 2024 6,688 3 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to cut a sample with several micrometers thick silicon oxide with DISCO DAD323 dicing machine?

I use a DISCO DAD323 dicing machine to cut my sample,which has 1-2 micrometers SIO2 on its top.However,the SIO2 is broken and fall apart everytime.I tried different cutting speed but didn't work....

20 June 2024 1,131 0 View

DNA FISH with multiple signal spot ?

I am doing DNA FISH on mice stem cells to detect an endogenous gene location. The expected result should have two signal spots, representing the two copies of the allele. However, what I see is...

06 June 2024 941 1 View

Question on cardiac tyrosine hydroxylase expression in western blotting?

Our western blotting for tyrosine hydroxylase consistently shows double bands (50-70 kDa) in murine hearts using the #AB152 antibody from Sigma-Aldrich. Has anyone else encountered this issue? If...

30 May 2024 4,308 2 View

To do the hanging drop cell culture, does the lid of the petri dish has to be treated?

Because so far, the petri dish we got are only treated at the bottom part of the petri dish not the lid. Can the spheroid still form?

21 April 2024 9,681 3 View

Why the temperature exceeds the defined max value in FLUENT simulation?

My FLUENT model includes fluid and solid regions, with Discrete-Ordinates (DO) radiation model. The heating boundary conditions are defined by a named expression of Temperature-Time for the...

16 April 2024 6,636 3 View

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Why results of ROS flurescence are negative as there was no bacteria within?

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