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Hello,
the simplest way is to stain cells with calcofluor white. Or extract cell wall chitin, acid hydrolysed, and them measure with HPLC.
I've sent my plasmids for whole plasmid sequencing using the Oxford nanopore sequencing method. All of them were assembled as monomers, but in the reads (see attached pictures), there are even...
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Last week, I transformed home-prepared E. coli TOP10 cells. When checking the colonies, I noticed that some of them have yellow color. They did not grow on the randomly, but dependently on the...
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I'm trying to use reinforcement learning with live EEG measurements. However, just 2000 measurements/iterations take 16.6 minutes to measure and it seems I need at least 10 hours of live...
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A friend did PCR and then loaded it on agarose gel. All samples were prepared in the same way; the PCR was identical, with the same template, only primers differed, the same amount loaded on the...
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Hi! I am trying to do some boxplots in graphpad. I would like to use the 5-95 percentile boxplot option, but if I choose this option I do not know how can I also see the individual values of each...
17 April 2024 6,093 0 View
I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...
09 April 2024 4,487 5 View
I was looking at a sediment sample from a "biosphere" that I´m creating and I came accross this little guy. I´m almost certain that it´s a freshwater diatom but would like to know the specific...
19 March 2024 1,763 10 View
I just found the Platinum SuperFi II DNA Polymerase, which should simplify PCR protocol as it allows uniform annealing temperature of 60°C, but it should also have very high fidelity; >300×...
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quisiera que me proporciones un sistema de gestion de calidad
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ME PUEDE PROPORCIONAR INFORMACION SOBRESistema de Gestión de Calidad Basado en la Norma ISO 9001:2015 para el Instituto de Educación Superior Tecnológico
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I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...
31 July 2024 3,333 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.
25 July 2024 9,976 3 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
In recent years, quantum computing has emerged as a groundbreaking technology with the potential to revolutionize various fields, including artificial intelligence (AI). AI has already made...
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Hi All I recently get admission in PhD statistics. After a long discussion with my supervisor, the topic I selected for PhD is " Air Pollution and its impact on Economy: A case study of...
15 July 2024 1,820 5 View