Any recent studies on Interferon-gamma,interleukins6,10 and hepcidin in PTB-HIV patients?

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What is the most effective method for knocking out a gene in Candida albicans?

I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...

15 July 2024 3,977 1 View

Can an average household DIY spot welder be used for pouch cell electrode tab?

Would an average spot welder suffice for welding tabs for pouch cell electrodes instead of an ultrasonic one? Why would an ultrasonic welder be better than a regular spot welder?

10 July 2024 8,763 0 View

How can i access papers on distributed generations and their impact?

The impact im looking for could be both positive and negative on the electric grid

18 June 2024 9,269 3 View

Assess How Data Analytics Tools Are Transforming Financial Decision-Making Process in Businesses?

09 June 2024 735 1 View

In designing and construction of electronics device what are the possible ethical issues ?

What are the major ethical issues to be considered while working on design and construction of electronics device in a research work.

03 June 2024 8,189 4 View

What are the likely variables of monetary policies and bank interest rate when conducting research with this terms?

The impact of monetary policy and bank rate on the performance of deposit money banks in Nigeria

31 May 2024 7,587 1 View

Hi everyone, please how do I map plasmids encoding ARGs on an assembled genome, and determine their transferability?

The result from PlasmidFinder shows that the assembled genome harbored many plasmids. Consequently, I want to investigate whether the plasmids are encoded for ARGs. And determine if they are on...

08 May 2024 7,467 0 View

Wi-fi and Li-fi possibility emerge?

ls it possible to emerge the wi-fi and Li-fi in one device and regular them.

21 April 2024 9,292 0 View

Can someone help me full MATLAB code using genetic algorithm to solve Economic dispatch problem?

The code needs to be able to work on a 28-bus system having cost as the objective function and constrain like transmission loss.

18 April 2024 3,349 1 View

Why do most people use GMM in analysis that focus on Africa?

I want to understand why most analyses conducted in Africa use GMM instead of other panel data estimation techniques.

16 April 2024 5,322 3 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

Does HEK293T cell express RIG1?

Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷

13 July 2024 3,382 0 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

30 June 2024 6,502 5 View

How do you bleed HIV-infected humanized mice?

Hello everyone, I usually use the saphenous vein to collect blood from HIV-infected mice (for viral load, CD4/CD8, etc.). However, I would like to know what methods other researchers are using to...

27 June 2024 7,707 2 View

Motivational systems bind perception and behavior?

It was gestalt psychologists that took issue with elementalism (Kohler 1929), that all perception/consciousness can be broken down into component parts. The expression ‘the whole is greater than...

16 June 2024 9,199 0 View

How do i use researchgate to cunduct literature review ?

I am a first-time researcher in the field of nursing and i would like to conduct research about knowledge and attitude towards prevention and spread of tuberculosis.

11 June 2024 3,821 2 View

Do you know a partner, friend, colleague, or someone else who was a Canadian gay (male) nurse who cared for PLWH during the HIV/AIDS pandemic?

My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...

10 June 2024 6,253 0 View

?How can we study proton radius with a 600keV high luminosity gamma ray ?? mark: gamma ray; proton; ?

Our gg collider will make a high luminosity gamma ray beam: 600keV, 10^9photon/pulse, 50Hz, small emittance angle:2.5mrad. We want to know how can use this gamma beam to study the characteristics...

03 June 2024 6,849 0 View

The gap in diagnosing intra-thoracic tuberculosis in children is not closed despite the advanncement in microbiology fields?

In 2024, childhood tuberculosis will still be a significant global public health issue, especially in underdeveloped nations (LMICS). It is true that the underestimation of the disease's extent...

31 May 2024 5,221 0 View

Does RAW 264.7 cells express MI like marker CD86?

I have come across literature that RAW 264.7 are M1 biased, do they express CD86 without LPS/ IFN stimulation?

27 May 2024 6,359 1 View