Dear, Guillermo Emilio Marcial There are several technique for isolation of essential oil from aromatic plants, such as hydrodistillation, steam distillation, etc. but for thermo-sensitive compounds containing essential oil Microwave assisted hydrodistillation technique is much more convenient.

https://www.newdirectionsaromatics.com/blog/articles/how-essential-oils-are-made.html

Extraction with non-polar solvent ( n-hexane or petroleum ether) using ultrasonic bath (sonication) at room tempreture.

thanks all for the replies! Now I really interested in the study of terpenes and sesquiterpenes avoiding heating process due to the strong oxidation of some compounds. For that, I would like to get any protocol using non-polar solvent using sanitation as replied Faiq H S Hussain. I would prefer the hydrodistillation, but with the equipment we have in our lab, the time under high temperature is longer than desire.

Regards

lyophilization then dissolving your extract in any suitable solvent,

or using any suitable distillation methods

No, but you can extract EOIs with dry and green process

Try with mixture hexane /plant material

If you want to carry out an extraction I would recommend pentane, because if there are very volatile substances in the oil, these can also be retained better when the solvent is removed. To achieve a certain polarity diethyl ether can be added, but be aware of stabilization agents.

Regards

Using separation by column chromatography at room temperature or below might be interesting.

Hi Guillermo, pentane/hexane is a nice solvent to obtain a (non-pure) essential oil Fractionation can be achieved easily by mini-column chromatography on silica gel. protocol see:

Article The Effect of the Volatile Oil from Ginger Rhizomes (Z ingib...

Hi Guillermo, use solid phase micro extraction (SPME) headspace at room temperature. Also, the solvent-free microwave extraction can be a useful technique.

Thanks for the comments and recommendations. They were really useful.

Regards

Kindly check this link, it contains useful information.

https://www.newdirectionsaromatics.com/blog/articles/how-essential-oils-are-made.html

1) Super critical CO2 extraction. Perhaps with hexane modifier. Expensive. Can scale to production.

2) Solvent Assisted Flavor Evaporation (SAFE) - Gentle technique applied to hexane, ether or similar extracts. Useful for separating waxes/fats from essential oil under vacuum. Essential oils are cryogenically trapped; residual solvent removable by Vigreux column. Less expensive, laborious, not suitable for high throughput. See widely cited reference below.

Article Solvent Assisted Flavour Evaporation (SAFE) – a new and vers...

SFE followed by small column chromatography in hexane/Pet ether is the most dutiable option.