02 January 2021 7 2K Report

OCT, US, FAG feautures

More Andreas Di Luciano's questions See All
Co-incubation of neutrophils with exosomes?

Hello! I am planning on doing co-incubation experiments of neutrophils with exosomes and wanted to ask what concentration of exosomes, cell number and incubation time works with this cell...

29 May 2024 6,551 0 View

Noves Ricerca Group?

Good morning to all, I have received an invitation to publish a paper from Noves Ricerca Group in a Q2 Journal. They have read my paper and very quickly they have accepted it for publication. It...

20 May 2024 7,407 3 View

What type of high-quality nanopure water do you use for NTA analyses?

We recently acquired a ViewSizer3000 from Horiba, and the manufacturer recommended us to use nanopure water from XZero for the NTA analyses. Do you recommend using any other brands of NW? Which...

05 May 2024 1,976 1 View

PCR & electrophoresis (confirmation) failing?

My fellow Academic colleagues! I together with my lab mates have a PCR-related issues that we hope that some(one) of you might have encountered and hopefully solved. In “short”, our initial PCR...

04 May 2024 8,788 5 View

What is the best way to concentrate the protein and remove the imidazole post Ni-NTA purification?

I've always struggled with concentrating and washing my proteins after purification. Often I lose almost everything in the process. I have used dialysis membranes followed by column filter...

16 April 2024 5,716 0 View

Orbitrap Astral Mass Spectrometer: applications?

Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...

10 April 2024 9,346 0 View

Has anyone experience with a good one antibody for the western blot detection of SQSTM1 in human cells?

The old antibody has been discontinued and the currently offered are detecting a band at 62 kDa, which does not reflect the correct molecular weight (47 kDa).

17 March 2024 4,300 2 View

Seeking Reviews for Q-TOF LCMS-9030 Mass Spectrometer?

Dear Forum Members, I hope this message finds you well. I am currently considering the purchase of a Q-TOF LCMS-9030 Mass Spectrometer for my laboratory, and I would greatly appreciate hearing...

10 March 2024 9,081 0 View

How can a false link be correcte?

When clicking on the publicatoin "Focal Cortex Stimulation With a Novel Implantable Device and Antiseizure Outcomes in 2 Prospective Multicenter Single-Arm Trials" by Schulze-Bonhage et al., the...

27 February 2024 5,522 0 View

Linear correlation between serum galactomannan and Aspergillus load?

Is there a direct relationship between the amount of galactomannan in the blood and the presence of Aspergillus in the lungs? In other words, does a higher level of galactomannan in the blood...

07 February 2024 9,725 0 View

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Tile of study is "Comparing Health Information-Seeking Patterns in Exceptional versus Normal Conditions" Objective of the study is "to examine the influence of contextual differences and...

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Calculate sample size in cross-sectional studies?

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Are there people who success to staining pax7 on mouse tissue (frozen, 4%PFA)?

I have never success a staining pax7, myoD, eMHY on mouse tissue. (DSHB antibody) When I did a heat-retrieval, staining is success but background was too strong. So I'm not sure if this is a good...

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How to calculate binding energy and different interactions from gaussian for halogen bonded co-crystals?

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What is the best approach for embedding and cryosectioning rodent optic nerves?

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