Adjust annealing temperature or additives to enhance specificity of PCR?

Dear All, I have a very basic question about the PCR/RT-PCR/qPCR. The PCR primers and TaqMan probes are designed such that the primers/probe connot bind to the mismatched site but only...

15 June 2020 4,926 3 View

Crystallization of PLLA?

07 June 2020 2,395 6 View

How to define grain boundary hardness?

08 March 2020 3,388 11 View

Will the TaqMan probe length affect the fidelity of the qPCR assay?

Dear all, I am learning to design the qPCR assays recently. But I just don't know why my negative controls always give positive signal( I have changed my design twice). I checked the formation of...

07 October 2019 4,968 3 View

Effect of mRNA secondary structure on reverse transcription?

Dear Sir/ Madam, I am designing a primer for microRNA reverse transcription. But some microRNAs like hsp-mir-21-5p has a strong secondary structure. It forms a stable hairpin. Can anyone please...

08 September 2019 8,283 3 View

How to deal with the nonlinearity in electrical tomography?

The electrical tomography can be utilized to calculate the solid concentration or gas holdup in aqueous based multiphase flows. However, there usually exists a strong nonlinear relationship...

16 May 2019 197 1 View

How many base-pairs can be added to the pSin vector?

Recently I decide to add a 5.5 kb fragment to pSin vector will it affects the efficiency of virus packaging?

20 March 2019 9,786 2 View

How can I deal with the non-specific binding in XIST-RNA-FISH?

I'm trying to detect the XIST-RNA expression in Breast cancer cells using the Biotin and Digoxin probes. However, there are always non-specific bindings in my results. I have added RNase-inhibitor...

18 March 2019 3,761 1 View

Any UV light absorbing material at 193-248 nm?

Would like to coat the alloy surface with non-organic material to absorb the UV light at 193-248 nm. Please recommend some UV absorbing material. Thanks~

16 November 2018 1,466 1 View

Annealing temperature set-up for short primer of 11 bases?

Dear all, I am amplifying a target sequence of 65 bases, which is a cDNA sequence now. For some reasons, my PCR primer can only bind to the target sequence with 11 bases in the first PCR cycle....

30 October 2018 802 7 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View