How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

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02 March 2021 932 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What role did digitalisation play in the oil and gas industry in the COVID-19 epidemic?

As known, the covid epidemic has made big changes in the work of entire sectors of the economy. In Russia, not so long ago, the process of digitalization in the oil and gas industry began, which...

16 December 2020 6,499 2 View

How To Run Granger Test with Panel Data? Do you have a practical guide?

Hello together, I would like to perform the Granger Test with my panel data. I am investigating 40 banks and examining the impact of digitization on ROA. Specifically, I have the variable...

01 December 2020 3,642 5 View

Hello everyone, does anyone have this attached map as a shapefile?

- I did georeferencing and digitizing for it, but it will be more accurate to have the original one.

19 October 2020 9,550 7 View

DNA studies from herbarium collections: which is the best commercially available DNA extraction protocol?

Herbarium collections are increasingly becoming enormous resource for DNA studies; but for many species in certain families, there is difficulty in obtaining amplifiable DNA. I would like to...

25 March 2020 4,469 11 View

Is there something I can do to increase the chances of successful ISSR bands amplification during PCR ?

Hello, I have a following issue: I extracted approx. 20 plant DNA samples from dry leaves (old herbarium items) using Qiagen DNeasy Plant Mini kit. When I run a PCR for housekeeping gene (alpha...

22 March 2020 2,664 6 View

What is the best method to extract and identify pigments from fruits obtained from herbarium specimens?

I'm searching for a specific pigment produced in the fruits of Lonicera spp. Although I can use fresh fruit, I would like to perform extractions from herbarium specimens. Any assistance will be...

12 February 2020 3,381 3 View

What is the best method to extract and identify pigments from fruits obtained from herbarium specimens?

I'm searching for a specific pigment produced in the fruits of Lonicera spp. Although I can use fresh fruit, I would like to perform extractions from herbarium specimens. Any assistance will be...

01 February 2020 7,235 3 View

How to barcode hybrid plants ?

Taxonomic identification of plants can be done by gene barcoding. There are several reported gene markers to be used to barcode unknown species. My question is "Is it possible to identify the...

23 January 2020 6,704 3 View

How can I locate global "remote" tenders?

Hi there, I hope you're doing well. I assume that there are some tenders that require that the applicants be from the same country of the tender. Is there a way to filter results where only...

28 December 2019 4,527 5 View

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30 October 2019 7,430 11 View