Hello,
do you have any experience using holographic microscopy in life sciences (cancer biology, cell death analysis, cell-cell interactions, virology, microbiology)? If so, what were the biggest disadvantages?
I assume the answer is needed to fill in the 'limitations of the study' section in the paper, right? Coming from the technology side -- and, admittedly, without any practical experience with applications in cell biology -- I would guess that the main disadvantage will be the fact that most of your measurement equipment will be experimental and hence lacking in proper measurement validation. Quite simply, with holographic microscopy you do not have a large enough library of reference images to support your claim about what is what.
Resolution as rule is lower, because of coherent noise (speckle).
Our time set up was cumbersome (we used film to make a hologram) and expensive.
See link
http://markoff5.tripod.com/Projects/biophotonics.html#NerveInterferogram
It is a special interferometric image. Holography was just an auxiliary tool in this microscope. Primary purpose was to get a high-frequency fringes and inexpensive interferometric image, but not 3D.
I believe I wont be mistaken much if I say that DHM is a comprehensive technique nowadays. Applied in coherent, partly coherent and absolutely incoherent light or better to say in any part of the electromagnetic radiation, ranging from X-rays up to far IR spectrum. Used whenever phase is essential or traditional optics can not be applied due to lots of reasons. Since you focus on biology and cells studies especially, I believe Biomedical Optics Express journal is one to start the search. I think I saw a lot of papers there on topics relating to your question.
I agree with George that it can be a tool to study phase objects.
Another holography advantage - 3D - is difficult to implement in microscopy. There is no room to record hologram before lens because of small space. If you record a wave after lens it is distorted by optical system and introduce all it's limitation like depth of field. New techniques are necessary to bypass this limitation.
Most important applications in biology and mems are those offered by digital holographic microscopy. characteristics include live dynamical 3D topography, 3d Vobration analysis (in-plane resolution 1 nlm (nano meter), out-of-plane resolution 5 pm (picometers)).
You may start at http://www.lynceetec.com/, then choose your way in function of your interest.
I hope it helps,
Dan
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