After 5 minutes of Chemiluminescence substrate incubation, the image was reversed. Any ideas on how to make it better?
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
I would like to hear if anyone here has had prior or present experience with Lightshift Chemiluminescent RNA EMSA kit from Thermo Scientific? What are the gels you have used to detect the labelled...
23 June 2024 7,463 1 View
Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...
13 June 2024 9,936 5 View
I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...
02 June 2024 7,833 2 View
We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...
25 May 2024 7,663 3 View
I have two questions: 1. After transfer, the marker confirmed that the transfer had proceeded well. However, I would like to know why only one protein band appears in the high kDa region when...
29 March 2024 1,830 0 View
I'm working on a project related to Luminescence Properties of Rare Earth Glasses (not Europium). I have read some articles related to Judd Ofelt Parameters, but I couldn't able to understand the...
26 March 2024 2,287 0 View
My student optimizes the RNA dot-blot method using a biotinylated cDNA probe. The aim is to detect a positive hybridization signal of a probe complementary to transcripts of a housekeeping gene...
17 March 2024 4,741 3 View
My transfer buffer Ph is 8.7 but should be a 8.3 is this causing my exposures to be off also my bands look smeared. i am trying to troubleshoot and figure out why my chemiluminescence western blot...
12 March 2024 7,433 1 View
Hi, I am interested in performing a reporter assay in organoids grown in matrigel. Is there a commercially available kit that would allow me to read both firefly and renilla luciferase...
06 February 2024 6,029 0 View
I am doing EMSA using LightShift Chemiluminescent RNA EMSA Kit from Thermo Scientific. For the detection, I use stabilized Streptavidin-Horseradish Peroxidase Conjugate and Chemiluminescent...
30 January 2024 2,314 1 View