I would like to do a study in vitro to evaluate any antioxidant activities to protect cells but I do not know if there is any study for the dilution for these extracts in order then to study them, or if anyone did a similar sttudy can suggest to me any protocol..the extracts are : oil obtained after SF-CO2, Aceton, Methanol and water in order.
Thanks a lot
There is not any protocol, first of all you must add plant extract without any traces of methanol and acetone, and after test the plant extrat at different concentrations and found the range more suitable for your study
I think 1% DMSO will be ok for the dilution, if it is water there wont be any need bcos it is not toxic to cells. There is a cell lines for antioxidant testing, check it out online.
Hi Husseen, I have published a number of papers investigating the antioxidant activity of seaweed extracts. Try diluting the extracts in water or 1% DMSO. Next you need to determine the possible toxicity of the extracts using the MTT and neutral red assay. These assays will give you the maximum concentration of extract that can be safely used in the cells. Have a look at my attached paper
Article The effect of solvents on the antioxidant activity in Caco-2...
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