Shalom  Noam,

Depending of the metabolites it would be possible to  work with standards and to do the evaluation of the treatments  by means HPLC-MS-MS, HPLC-PAD in order to compare the heterogeneity. It would be important to define the behavor of the metabolites both peels and pulp of the fruit due to the accumulation could display a meaningful variability.

Marcela Castro-Benitez

Gracias Marcela,

I'm using a UPLC-MS-MS and GC-MS systems to separately analyzing the skin and pulp tissues. I refere to the heterogeneity between same tissues sampled from different treatments. What would be a correct statistical approach? 

Shalom Noam,

Because you have differents treatment and analyzed the skin and pulp tissues, I could you recommend a factorial experimental design  for a correct statistical approach. i.e. (2x2) refered  skin and pulp tissues as (2) and two treatment (2). Whether you analyze differents kind of fruit, then you should add an additional factor with its respective number of levels.