Any experience using RNAseq for profiling interferon expression?

14 March 2017 0 7K Report

We are considering this technique but given its expense are not sure of its benefits as compared with simple ELISA

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What is wrong with my calculations? (MDA equivalents in the TBARS assay)?

Hello, I am trying to MDA equivalents as described by Hodges (1999), but I am having trouble determining if I am doing the proper calculations. The authors provide the following equation: MDA...

12 June 2022 9,640 0 View

How to interconvert from millimolar extinction coefficient to molar extinction coefficient?

Hello, I am working with the TBARS assay and doing absorbance readings of malondialdehyde (MDA) at 532 nm wavelength. The literature reports the extinction coefficient of MDA in several different...

12 June 2022 2,593 1 View

Best way to attatchment of HEK 293T cells to plates for immunofluorescence screening?

I am trying to screen HEK293T cells by immunofluorescence and even though I coat the 96 well plates with poly-l-lysine (pll)after multipe washes (being very careful) there are few cells left. Has...

10 April 2022 3,184 2 View

What is the best SPSS test to carry out for the question in the description?

I need to find out whether "gender influences the effectiveness of the two intervention programmes designed to increase participants confidence in being able to cope with statistics training?"....

26 December 2021 3,043 3 View

Are there any available doctoral level supervising Professors within the field of the Circular Economy here?

Would be interested in a discussion with Professors within the field of the Circular Economy actively conducting research presently and with doctoral students (now/future students).

19 April 2019 1,088 1 View

Does Clostridium carboxidivorans have a native plasmid?

20 November 2018 2,840 3 View

Re-alignment of HPLC MS spectra data, is it possible?

Dear Forum Is there any way to rescue HPLC-MS chromatographic data that has shifted mid run to another RT? The quantitation was not affected. However PCA analysis of the data gave a false positive...

10 November 2018 6,308 2 View

Can anyone point me to papers that discuss blue mussel feces egestion rates under different conditions?

I'm trying to do an input/outputs budget for some experimental contamination work I conducted but I can't find any information on average feces egestion rates in mussels.

01 March 2018 9,922 1 View

Book citation for in Mendeley with Editors but no Authors?

Entered into Mendeley as folllows Type: Book Dictionary of Alkaloids Authors: "blank" Year: 2010 Volume: "blank" Issue:"blank" Pages:"blank" Abstract: "blank" Tags: "blank" Author Keywords:...

10 November 2017 5,544 2 View

Is ferroelectricity always anisotropic?

Forgive me if my question is naive, but I'm hoping to gain some clarity in describing ferroelectric phenomena. The assumption I'm making in my statement is that if a ferroelectric crystal must...

28 October 2017 9,387 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (E-ISSN 1901-9726, P-ISSN : 0900-9671) a legitima?

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (ISSN Online: 1901-9726, ISSN Print: 0900-9671) a legitimate and credible scholarly journal in the field...

01 August 2024 629 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Will combining different research methods help in my case?

Dear colleagues, I wonder if combining different research methods, namely literature review, semi-structured interviews and structured questionnaire survey will help to ensure validity and...

30 July 2024 5,154 17 View

In terms of chaos, what is the necessary and sufficient condition for authoritarianism, permanent or temporary, to come to exist and persist?

Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...

28 July 2024 6,515 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View